WebProduct overview. Abcam’s BCA protein assay kit reducing agent compatible (microplate) (ab207003) measures total protein concentration of pure proteins, extracts or lysates in the presence of reducing agents. Unlike the majority of protein assay kits, ab207003 is compatible with strong reducing agents such as TCEP up to 20 mM, DTT … WebQuantify protein quickly and accurately with two unique Qubit protein quantification kits: the Qubit Protein Assay, which quantifies 12.5 µg/mL to 5 mg/mL of protein, and the Qubit Protein BR Assay Kit, which quantifies 0.1 to 20 mg/mL of protein. Both assays are compatible with many common contaminants, reducing reagents, salts, and nucleic ...
NanoOrange™ Protein Quantitation Kit, 200-2,000 assays
WebJun 27, 2024 · Originally developed in 1985, the BCA assay is another colorimetric assay. (3) This two-step assay first makes use of the biuret reaction, in which the protein backbone chelates Cu 2+ ions and reduces them to Cu + ions. In the second step, the Cu + ions react with BCA to form a purple-colored product that absorbs at 562 nm. WebI need quantify the protein amout from human DCs lysed by RIPA buffer ( 150 mM NaCl, 1% NP-40 or Triton X-100, 0.5% sodium deoxycholate, 0.1% SDS, 50 mM Tris, pH 8.0 ) to use in a immunoblot. Some ... kites unlimited beaufort nc
DC Protein Assay Bio-Rad
WebThe EZQ™ Protein Quantitation Kit provides a fluorescence-based protein assay that facilitates fast quantitation of protein samples prepared for gel electrophoresis. The … WebMRM Proteomics Inc. offers an extensive line of bioassay kits that enable users to perform protein quantitation using their own in-house LC-MS/MS instrumentation. Our complementary line of quality control products will help to ensure that your proteomics workflow and instrumentation is up to standard for every sample you analyze. WebQuant-iT™ Protein Assay Kit 2 Table 1. Mass-to-Concentration Conversion.* Protein Mass (µg) Final Concentration µg/mL Concentration (µg/µL) in Sample Volumes of 1 µL 10 µL 20 µL 0.25 1.25 0.25 0.025 0.012 1 5 1 0.1 0.05 5 25 5 0.5 0.25 * The Quant-iT protein assay is designed to detect 0.25–5 µg of protein in a 200 µL assay volume. magazine manager software