WebTHP-1 cell line (ATCC TIB-202) is commonly used to study monocyte and macrophage activities, functions, innate immune mechanisms and signaling pathways. This luciferase reporter cell line was derived from parental line TIB-202 by stably expressing firefly luciferase gene (luc2) under control of a NF-κB promoter. This cell line was established … WebTHP1-Dual™ Cells. Catalog number. thpd-nfis. Price. Please ask . Size. 3-7 x 10e6 cells. Test. The duality of this product lies in it's dual specificity to the gene, pH or protein. Mainly in phosphorylation by specific phosphatases. Gene target. THP1 Cells . Gene symbol. GLI2. Short name. THP1- ™ Cells. Label. dual. Alternative name.

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WebOct 16, 2024 · THP1 Dual reporter cells were seeded into the culture wells, and after 24 h, culture supernatants were collected for NF-κB and IRF reporter assays. RNA extraction and qRT-PCR RNA was isolated from cells (300,000 cells/well; 24-well plates) at the indicated time points after stimulation using the NucleoSpin RNA kit (Machery–Nagel) or with … WebTo determine whether the loss of IFN induction from mRNA in the MAVS −/− THP1-Dual cells was secondary to an impact on the TLR7/8 pathway, the mRNA preparations were also tested in MyD88 − ... inglehoffer extra hot horseradish 4 oz jar

THP-1细胞培养经验与要点 - 知乎 - 知乎专栏

WebArticle Snippet: THP-1 (DSMZ) and THP-1 NLRP3 deficient (N def ), THP-1 Caspase-1 deficient (Casp1 def ), THP-1 NLRP3 knockout (N ko ), THP-1 ASC::GFP, THP1-Dual, and THP1-Dual-TLR4 KO Cells (all Invivogen) were maintained between 0.5-1×10 6 cells/ml in RPMI 1640 media, supplemented with 10% Fetal Bovine Serum and 100U/ml penicillin, … WebJan 31, 2024 · Reporter Cell Line > Product List InvivoGen에서는 실험 목적에 맞는 다양한 reporter cell line을 제작하여 공급 해드리고 있습니다. Microbial molecules, cytokine에 의해 유도되는 세포 내 signaling pathway의 activation을 확인할 수 있는 세포입니다.이러한 engineered cell line들은 SEAP and/or Lucia luciferase reporter gene의 발현이 stable ... WebApr 9, 2024 · THP1-Dual™ KI-hSTING-S154细胞和THP1-Dual™ KI-hSTING-M155细胞均来自THP1-Dual™ KO-STING细胞，该细胞衍生自THP-1细胞，稳定敲除了内源性人源STING的双等位基因并稳定整合了两种可诱导分泌的报道基因（Lucia萤光素酶和SEAP（分泌型胚胎碱性 … mitsubishi electric halle düsseldorf